Interstrand-cross-link repair
WebDrugs that produce DNA interstrand crosslinks (ICLs), between the two complementary strands of the double helix, have an important role in chemotherapy regimens for cancer. … WebThus, repair mechanisms of lesions caused by ICLs may cause point mutations as well as deletions and translocations leading to tumor growth 3. The ICL repair mechanism has …
Interstrand-cross-link repair
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In genetics, crosslinking of DNA occurs when various exogenous or endogenous agents react with two nucleotides of DNA, forming a covalent linkage between them. This crosslink can occur within the same strand (intrastrand) or between opposite strands of double-stranded DNA (interstrand). These adducts interfere with cellular metabolism, such as DNA replication and transcription, triggering cell death. These crosslinks can, however, be repaired through excision or recombinati… WebHere we exploit the simple eukaryote Dictyostelium to uncover a role for ADP-ribosylation in regulating DNA interstrand crosslink repair and redundancy of this pathway with non-homologous end-joining (NHEJ). In silico searches identify a protein that contains a permutated macrodomain (Aprataxin/APLF-and-PNKP-Like protein; APL).
WebSep 4, 2001 · Mammalian DNA interstrand cross-link repair7.1. Finding genes involved in mammalian DNA interstrand cross-link repairKnowledge about ICL repair in mammalian … WebRemoval of a DNA interstrand crosslink (a covalent attachment of DNA bases on opposite strands of the DNA) and restoration of the DNA. DNA interstrand crosslinks occur when …
WebInterstrand crosslink (ICL) recognition by FANCM and associated proteins leads to the recruitment of the Fanconi anaemia (FA) core complex and monoubiquitylation (Ub) of … WebOct 1, 2013 · Cross-link structure affects replication-independent DNA interstrand cross-link repair in mammalian cells. Biochemistry 49: 3977–3988. [Europe PMC free article] [Google Scholar] Ho TV, Schärer OD. 2010. Translesion DNA synthesis polymerases in DNA interstrand crosslink repair. Environ Mol Mutagen 51: 552–566. [Google Scholar]
WebDNA is subject to a wide variety of endogenous and environmentally induced modifications, including base oxidation, deamination, depurination and formation of interstrand cross-links (1). The ...
WebJul 2, 2015 · INTRODUCTION. Interstrand cross-links in cellular DNA are highly deleterious because they prevent the strand separation that is necessary for cellular machinery to extract genetic information from the double helix during transcription and replication. 1–3 We recently characterized two new structural types of interstrand cross-links derived from the … luxthos weakaura warriorWebJan 23, 2016 · Fanconi anemia (FA) is a hereditary disorder characterized by bone marrow failure and genome instability that is ascribed to defective DNA interstrand cross-link (ICL) repair. In this chapter we summarize our current understanding of the function of the FA genes, the mechanism for FA pathway activation, and the processes of ICL repair in the … jean white steventonWebMay 17, 2024 · Generally, haematological cell lines are more sensitive to SG3199 than solid tumor cell lines. Moreover, SG3199 forms DNA interstrand cross-link in both naked DNA and cells. Furthermore, compared with CHO wild-type AA8 cells, cells defective in DNA repair protein ERCC1 or homologous recombination repair showed increased sensitivity … jean whitemanWebJul 1, 2011 · ICL repair requires RAD51-dependent HR. (A) Cross-linked sperm chromatin was replicated in extract containing buffer (Mock) or the indicated BRC peptide, and chromatin-associated proteins were ... luxthousWebDrugs that produce DNA interstrand crosslinks (ICLs), between the two complementary strands of the double helix, have an important role in chemotherapy regimens for cancer. Novel crosslinking agents, and targeting strategies involving DNA crosslinking agents, continue to be developed. The ability of cells to repair DNA ICLs is a critical determinant … jean white tarboro ncWebDuring eukaryotic DNA interstrand cross-link (ICL) repair, cross-links are resolved (“unhooked”) by nucleolytic incisions surrounding the lesion. In vertebrates, ICL repair is triggered when replication forks collide with the lesion, leading to FANCI-FANCD2-dependent unhooking and formation of a double-strand break (DSB) intermediate. luxthos weakauras warlock wrathWebJul 29, 2024 · The HMCES protein forms a covalent DNA-protein cross-link (DPC) with abasic (AP) sites in ssDNA, and the resulting HMCES-DPC is thought to suppress double-strand break formation in S phase. However, the dynamics of HMCES cross-linking and whether any DNA repair pathways normally include an HMCES-DPC intermediate … luxthread