Pdgfb-creert2

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August undefined, 2024

Splet01. maj 2024 · Pdgfb‐iCre ERT 2;JAK2 V617F/ WT mice develop a myeloproliferative disease 10 weeks after induction of JAK2V617F expression. Conditional flexed JAK2 … Splet10. jan. 2013 · In this review, we described several Cre lines that target ECs, including Tie2-Cre, VE-Cadherin-CreERT2, PDGFB-CreERT2, and others. Many of these Cre alleles target all ECs, and therefore it would be helpful to generate Cre alleles and CreERT alleles that specifically target CNS ECs. To do so, one could take advantage of the split-Cre system.

Comparison of endothelial promoter efficiency and

SpletFumiaki Uchiumi, in Bidirectional Gene Promoters, 2024. Growth factors and receptors. The PDGFB (c-sis) [12] and CSF1R (c-fms) [13] are the earliest identified proto-oncogenes, … SpletPlatelet-derived growth factor (PDGF) and its receptor play an important role in embryogenesis. PDGF receptor α (PDGFRα) is expressed specifically in the embryonic … toy fairy catchers

Adult-induced genetic ablation distinguishes PDGFB roles in blood …

Splet19. feb. 2024 · We engineered these viruses to coexpress CreERT2 with PDGFB, allowing for deletion of floxed genes specifically in transduced cells, and designed another version … SpletThe targeting vector used to modify PAC 546-M3 is illustrated (a). The construct was designed to insert the tamoxifen-inducible form of Cre recombinase (CreERT2) (Indra et al., 1999) into the first coding exon of the Pdgfra gene (exon 2). Homology regions 0.5 kb in length were amplified by PCR from the genomic PAC using Expand High Fidelity ... Splet一、品系介绍： Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。 Cre-ERT2在无Tamoxifen诱导的 … toy fairy house

Tamoxifen诱导Cre-ERT2小鼠 cre小鼠品系介绍南模生物

Splet27. jul. 2024 · PDGFRβ-P2A-Cre T2 mice (recently deposited at The Jackson Laboratory as Stock No. 030201) were generated by replacing the translation stop codon (TAG) of the … Splet20. jan. 2024 · The Pdgfb-CreER strain was crossed with a Nrp1-floxed strain to produce offspring with or without the CreER transgene in a homozygous Nrp1-floxed background. Upon tamoxifen treatment, Nrp1 protein was depleted in choroidal endothelial cells (arrows in Fig 1) in CreER positive (Nrp1 ΔEC) but not in CreER negative (Wt) animals ( Fig 1G–1J ). toy fairy castleSplet10. jan. 2013 · In this review, we described several Cre lines that target ECs, including Tie2-Cre, VE-Cadherin-CreERT2, PDGFB-CreERT2, and others. Many of these Cre alleles target all ECs, and therefore it would be helpful to generate Cre alleles and CreERT alleles that specifically target CNS ECs. To do so, one could take advantage of the split-Cre system. toy fakemon

"Splet20. jun. 2024 · Pdgfb-CreERT2-mediated expression of Taz S89A (Taz iEC-GOF) in the retinal endothelium resulted in increased levels of nuclear TAZ as well as increased … " - Pdgfb-creert2

Pdgfb-creert2

Comparison of endothelial promoter efficiency and specificity

Splet20. jan. 2024 · Crossing Nrp1 floxed mice with Pdgfb-CreERT2 mice produced tamoxifen-inducible, endo- ... In order to confirm that the Pdgfb-CreER strain can drive Cre recombination in the adult cho-roidal ... Splet01. apr. 2013 · In b3-floxed/Pdgfb.Creert2 positive mice treated with tamoxifen, allograft tumours grow significantly smaller when compared to their growth in Cre negative littermate controls.

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Splet01. apr. 2016 · To test this proposed mechanism in adipose tissue, we deleted Flt1 in endothelial cells of Flt1-fl/fl;Pdgfb-CreERT2 (Flt1-EC KO) mice by tamoxifen administration (Figures S10A–S10D). This resulted in reduced levels of VEGFR1, with the remaining protein in adipose tissue and serum likely produced by non-endothelial cells such as macrophages. Splet25. okt. 2024 · The Pdgfb retention motif knockout mouse (Pdgfb ret/ret) is a hypomorphic Pdgfb model that has been used to study postnatal effects of pericyte loss, particularly in …

Splet16. nov. 2024 · Abstract. Restoring blood flow after myocardial infarction (MI) is essential for survival of existing and newly regenerated tissue. Endogenous vascular repair processes are deployed following injury but are poorly understood. We sought to determine whether developmental mechanisms of coronary vessel formation are intrinsically reactivated in ... SpletCre-mediated recombination occurs in a small number of adult hematopoietic stem cells in Pdgfb-iCreERT2;JAK2 V617F/WT transgenic mice. SUMMARY: Background The vessel …

Splet29. maj 2024 · The Tgfbr2flox/flox 22, PDGFB-CreERT2 20, and Rosa26-loxSTOPlox-YFP 22 mouse strains (on a C57Bl6/129S4 genetic background)have been described elsewhere. Pups (P1-P3) were injected in the stomach ... Splet029684 - Pdgfr-β-CreER [T2] Strain Details. Technical Information Scientists. Overview.

Splet13. apr. 2024 · Platelet-derived growth factor (PDGF) signaling is frequently aberrantly activated in GBM, primarily due to the amplification of the PDGF-receptor alpha ( PDGFRA) 25, 26 (Supplementary Fig. 2a ).

Spletthe PDGFB-CreERT2 line (23). Single deletion of Myh10 in endothelial cells (MIIB EC-KO) did not significantly affect retinal vascular morphogenesis, although we noticed a small decrease in the number of filopodia in endothelial tip cells (Sup.Fig.3). Yet, single deletion of Myh9 (MIIA EC-KO) showed a very distinct phenotype. toy famasSplet25. okt. 2024 · Platelet-derived growth factor B (PDGFB), originally identified as a constituent of PDGF molecules in blood serum and human platelets, is one of four PDGF chains (PDGF A-D) that make up the PDGF family of homo- and heterodimeric ligands. 1 The Pdgfb gene consists of seven exons. toy fake teethSpletThe Tg (Pdgfb-icre/ERT2) 1Frut allele was created by insertion of Cre/ERT2 coding cDNA at the start codon of the Pdgfb gene in a PAC, which was … toy fake moneySplet04. apr. 2013 · In b3-floxed/Pdgfb.Creert2 positive mice treated with tamoxifen, allograft tumours grow significantly smaller when compared to their growth in Cre negative littermate controls. toy fake foodSpletAltogether the results obtained with mT/mG reporter mice suggest, as previously published, that Cre is not expressed in the hematopoietic lineage of adult inducible Cdh5 (PAC)-CreERT2 and Pdgfb ... toy fallSplet06. jan. 2016 · Pdgfb-creERT2-induced overexpression of MYC caused sustained vascular overgrowth and led to a profound increase in EC number, proliferation and vessel density … toy falconSplet032770 B6.129S- Pdgfra tm1.1(cre/ERT2)Blh /J Pdgfrα-CreER T2 mice have a cre/ERT2 fusion gene inserted into the start codon of the platelet derived growth factor receptor, … toy fairy wand